A Rapid and Sensitive Antigen Capture Test for the Detection Specific Cells on Shear Horizontal Surface Acoustic Wave Sensors

Cell separation techniques are used widely in immunology studies. The one-step antigen-specific separation technique on a microfluidic platform based on shear horizontal surface acoustic wave (SH-SAW) sensors. With the specific antibodies conjugated onto the surface of the SH-SAW sensors could be used to specifically recognize the specific cells in the clinical body fluids. The cell isolation and purification studied targeted cells (Jurkat), as a model for low abundance cells (1 : 1,000), with more dilute cells as the ultimate goal. T cells were successfully separated on-chip from the mixed cell medium (Jurkat cells/ K562 cells, 1/1000). The novel micro-chamber was developed for capturing process in the purification process when a relatively large amount of bio-sample was required. In addition, successful demonstration for detection of cells had been achieved by performing an on-chip genetic identification.